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. 2015 May 28;5:10475. doi: 10.1038/srep10475

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Copyright © 2015, Macmillan Publishers Limited

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A) SAP designates the SAF- A/B, Acinus and PIAS motif, while BBS denotes BRD7-binding site. The RGG/RG motifs located from 609 to 658 are shown. The RG and RGG repeats are bold and underlined. B) The human hnRNPUL1 peptide sequences fused to recombinant GST used for the methylation assay of panel C. C) PRMT1 in vitro methylation assay using GST-hnRNPUL1 proteins of panel B with H³-SAM. Proteins were resolved by SDS-PAGE, stained with Coomassie blue (left panel), dried and visualized by fluorography (right panel). GST-MRE11:RGG (glycine arginine rich) and GST were used as the positive and negative control, respectively. The indicated sequences (RGG or RG) fused to GST migrate at a similar molecular mass as GST alone because of the short added sequence. GST-MRE11:RGG harbors an extra ~60aa and migrates close to 33 kDa. The migration of GST-PRMT1 is shown with arrows.