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. 2015 May 28;5:10475. doi: 10.1038/srep10475

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A) Whole cell lysates from HEK293 cells transfected with siControl (CTL) and siPRMT1 were subjected to immunoprecipitation (IP) 48 h post-transfection with the anti-hnRNPUL1 antibody. Whole cell lysates (WCL) and immunoprecipitants were immunoblotted with anti-asymmetrical dimethylarginines (ASYM25b), anti-hnRNPUL1, anti-PRMT1 and anti-Tubulin antibodies. B) HEK293 cells transfected with wild type FLAG-hnRNPUL1 and FLAG-hnRNPUL1^RK were lysed and anti-FLAG immunoprecipitations were performed. The bound proteins were separated by SDS-PAGE and immunoblotted with the indicated antibodies. Tubulin was used as loading control.