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. 2015 May 28;5:10475. doi: 10.1038/srep10475

Figure 3. Methylation of hnRNPUL1 is required for its interaction with NBS1.

Figure 3

A) U2OS cells were transfected with empty vector (pcDNA 3.1), FLAG-hnRNPUL1 wild type, FLAG-hnRNPUL1RK mutant and YFP-NBS1. Whole cell lysates (WCL) and FLAG-immunoprecipitants were immunoblotted with the indicated antibodies. B) HEK293 cells were transfected with siControl and siPRMT1 along with YFP-NBS1 and FLAG-hnRNPUL1. The whole cell lysates (WCL) and FLAG immunoprecipitates were subjected to western blot analysis using the antibodies indicated.