Figure 4. RGG/RG motif regulates GFP-hnRNPUL1 recruitment at breaks.

A) U2OS cells were transfected with wild type GFP-hnRNPUL1 and GFP-hnRNPUL1^RK and subjected to laser micro-irradiation treatment (forms a DNA damage ‘track’; white box). Exclusion of GFP-UL1 proteins from laser damage sites (2 min post-laser scissor damage) was monitored via live imaging (exclusion) or recruitment in the presence of DRB (inclusion) B) Quantification of exclusion and inclusion patterns of GFP-UL1 proteins are indicated as a percentage.