Figure 1. Activation of GABA_B receptor upregulates Fmrp expression in CGNs.

(A) Fmrp expression in CGNs treated with indicated concentrations of baclofen. ***P < 0.001 vs. basal levels. (B) Fmr1 mRNA expression upon treatment with baclofen. (C) Time course of Fmrp expression induced by baclofen and CGP7930. Fmrp expression level was quantified based on three independent experiments (mean ± SEM). ***P < 0.001 vs. basal levels. Fmrp ratio and Fmr1 mRNA ratio were defined as the ratio between the density of each band and the sum of the densities of all the bands in a given blot. Full-size blots are shown in Supplementary Figure S1 and the band of interest is indicated by a red box.