Figure 7. IGF-1R and PKC are involved in GABA_B receptor-mediated Fmrp synthesis.

(A) CGNs were pretreated with AG1024 followed by treatment with baclofen. Fmrp levels were detected by western blotting and Fmrp ratio was defined as in Fig. 1A. Data represent the mean ± SEM from three independent experiments. (B) MEFs were co-transfected with GABA_B1, GABA_B2, and control or IGF-1R siRNA and then treated with baclofen. Fmrp levels were quantified as in panel A. (C, D) CGNs were pretreated with GF109203x or PF573228, and then treated with baclofen. Fmrp levels were detected as in panel A. Data represent the mean ± SEM from three independent experiments. For results in A, C, D, **P < 0.01, ***P < 0.001 vs. basal level; ^##P < 0.01, ^###P < 0.001, vs. baclofen-treated group. (E) CGNs were treated with vehicle, baclofen, CGP7930, GS39783, or Rac BHFF and Fmrp expression level was quantified as in panel A. Data represent the mean ± SEM from three independent experiments. ***P < 0.001, ns, not significant vs. basal level. Full-size blots are shown in Supplementary Figure S11 and the band of interest is indicated by a red box.