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. Author manuscript; available in PMC: 2015 May 28.
Published in final edited form as: Mol Cell. 2009 Jul 10;35(1):37–47. doi: 10.1016/j.molcel.2009.06.008

Table 3.

Kinetic parameters for subunit joining with all initiation factors in the presence of non-hydrolyzable GTP analogs.

# Molecules Intersubunit FRET events
Total # Uncensored (%) Mean lifetime (s) Mean arrival time (s)
All IFs - GTP 417 577 39 115.3 17.8 ± 2.0
Molecules with FRET on to FRET off fluctuations 48 117 57 45.7 12.6 ± 4.6
Molecules without FRET fluctuations 369 460 35 119.4 18.4 ± 2.1
All IFs - GDPNP 261 404 56 45.9 18.0 ± 2.5
Molecules with FRET on to FRET off fluctuations 72 182 67 27.8 17.7 ± 5.3
Molecules without FRET fluctuations 189 222 48 66.1 18.1 ± 3.0
All IFs - GTPγS 145 225 44 104.2 16.7 ± 3.4
Molecules with FRET on to FRET off fluctuations 22 59 56 54.7 11.0 ± 7.4
Molecules without FRET fluctuations 123 166 40 144.7 17.2 ± 3.9