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. Author manuscript; available in PMC: 2015 Nov 4.
Published in final edited form as: Virus Res. 2014 Aug 30;192:92–102. doi: 10.1016/j.virusres.2014.08.015

Figure 7.

Figure 7

Silencing of STX5 inhibited HIV-1 replication prior to HIV-1 integration. A) RNAi-mediated silencing of STX5 inhibited STX5 expression as measured by qRT-PCR compared to control shRNA treated cells (±S.D.) (N=3, p<0.007, two-tailed t-test). B) Silencing of STX5 inhibited HIV-1 replication as measured by reduced intracellular HIV-1 p24 levels and analysis by flow cytometry. C) STX5 depletion blocks HIV prior to viral integration. The targeted silencing of STX5 significantly impaired viral integration as measured by an Alu-LTR nested PCR strategy. Percent integration indicates percent versus Control shRNA treated cells (±S.D.) (N=3, p<0.03, two-tailed t-test). All values were normalized to β-globin. D) Late RT product formation was significantly inhibited upon silencing of STX5. Percent late RT formation relative to Control shRNA-treated cells (±S.D.) (N=3, p<0.03, two-tailed t-test). All late RT product values are normalized to mitochondrial DNA.