Figure 1. KLF-4 deletion impairs development of bone-marrow pre-cDC progenitors.

(A) Relative expression of Klf4, Irf8, and Irf4 determined by microarray analysis is shown for the indicated stages of DC progenitors. (B) Relative expression determined by microarray analysis of the indicated genes is shown for splenic CD24⁺ Sirp-α⁻ CD11b⁻ (CD24) and CD24⁻ Sirp-α⁺ CD11b⁺ (CD11b) DCs. (C,D) DC progenitors in BM were analyzed from wild type mice (WT), Klf4^fl/fl (cre-neg) or Klf4^fl/fl mice crossed onto Vav1-icre (Harker et al., 2002), or Irf4^fl/fl mice crossed into B6.C-Tg(CMV-cre)1Cgn/J (The Jackson Laboratory) backgrounds. CDP, MDP and pre-cDC were gated as described (Liu et al., 2009) in the Methods and analyzed for the indicated markers. (E) The percent of the progenitors from the indicated genotype analyzed in (C,D) are shown. The experiment was repeated 4 times and 2 mice per genotype were analyzed Error bars, ± s.d., n = 5, Student’s t-test. *P < 0.05; ***P < 0.001; NS, P > 0.05. See also Supplemental Figure S1