Figure 5.

Calibration of concentration-dependent optical NP response with liposomes. a) (I_scat, λ_res) scatter plots for liposomes with varying PS (top row) or G_M1 (bottom row) concentrations as specified after labeling with gold NPs. Data are plotted as black markers, and fitted distribution functions, P(I_scat, λ_res), are overlaid as color maps. The white dashed lines comprise the I_scat × λ_res, area, A_90%, that contains 90% of the data points. Each plot contains 500-1500 liposomes in total. b) Fraction, F, of labeled liposomes as a function of PS (top) and G_M1 (bottom) concentration. c) Plot of the spectral function B = F × A_90% for G_M1and PS. d) B versus F scatterplot for various liposome and VLP measurements. The data plotted in (a) – (c) were collected in three independent experiments.