TABLE 1 .
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Description | Reference |
|---|---|---|
| E. coli strains | ||
| DH5α | Molecular cloning strain | 30 |
| RH03 | Conjugation strain; Kms; DAP auxotroph | 39 |
| B. bronchiseptica strains | ||
| RB50 | “Wild-type” Bordetella bronchiseptica complex I strain | 14 |
| RB50 Kmr | RB50 containing constitutively expressed nptII inserted via a pUC18-based plasmid at the attTn7 site | This study |
| RB63 | RB50 containing deletion of the fimBCD operon | 20 |
| RB63 Gmr | RB63 containing constitutively expressed aacC1 inserted via a pUC18-based plasmid at the attTn7 site | This study |
| SP5 | RB50 containing an in-frame deletion of codons 227 to 756 of prn | 22 |
| RBX9f | RBX9 with a deletion mutation of the fimA-fhaB intergenic region | 31 |
| RB515 | RB50 containing a deletion in cyaA | 27 |
| WD3 | RB50 with an in-frame deletion of bscN | 40 |
| RB54 | RB50 Bvg− phase-locked variant with an in-frame deletion of bvgS | 14 |
| AS16 | RB63 with a mutation of the fhaB-MCD | This study |
| AS16 Gmr | AS16 containing constitutively expressed aacC1 inserted via a pUC18-based plasmid at the attTn7 site | This study |
| Plasmids | ||
| pSS4245 | pBR322-based allelic exchange plasmid; Apr Kmr | 30 |
| pTsS3 | Tn7 transposase expression vector containing tnsABCD; Apr | 41 |
| pUC18-miniTn7 | Transposition vector; Apr Kmr | 41 |