FIG 4 .

Imipramine and compound 3.47 inhibit filovirus replication and GP-mediated entry in vitro. Filovirus infected HUVEC and Vero cells were treated with or without imipramine (A) or compound 3.47 (B), respectively. Cells were fixed at 48 or 72 h postinfection, and infected cells were enumerated using MARV-, EBOV-, or SUDV-specific monoclonal antibodies and a fluorescently labeled secondary antibody. (C) Vero cells were treated with compound 3.47 at the indicated concentrations prior to infection with GFP-expressing VSV pseudoviruses. Cells were fixed 16 to 24 h postinfection, and infected cells were assessed by eGFP expression analysis. *, P ≤ 0.05; ***, P ≤ 0.001.