FIG 4 .
β-Galactosidase expression from ebpA::lacZ translational fusions is dependent on the identity of the start codon. (A) Schematic representation of the ebpA::lacZ fusions pTEX5749 (pSD2-ebpAATT::lacZ) and pTEX5750 (pSD2-ebpAATG::lacZ) carrying ATT and ATG as the start of EbpA::LacZ fusion protein synthesis, respectively. (B) β-Galactosidase activity in E. faecalis OG1RF and its ebpA start codon derivatives, TX5731 and TX5732, containing either pTEX5749 (gray bars) or pTEX5750 (green bars) after growth to mid-log phase in TSBG. Bars represent the means ± standard deviations of results from four independent assays, each with two duplicates. The mean values were compared using ANOVA with Bonferroni’s posttest (***, P ≤ 0.001; ns, P > 0.05).