Table 1. Primers and probes used in RT-PCR.

Target	Primer and probe sequence		Primer Concentration (nM)
Atrogin-1	F	5’ ATG CCG TTC CTT GGT CAG 3’	150
	R	5’ ACT GCT GAG GTC GCT CAC 3’	150
	P	5’ TGC CGC TTT TCT CAT CCA 3’	100
MuRF-1	F	5’ AGG ACT GAA TTT GTG TTA TAT GTT G 3’	150
	R	5’ TAG CCT CGA ACT CAT AGA GAT C 3’	150
	P	5’ AAC TGC CTC TGC CTC CA 3’	100
Myostatin	F	5’ AGA CAA CTT CTG CCC AGA G 3’	50
	R	5’ TCC GTG GTA GCG TGA TAA TC 3’	500
	P	5’ CCG TCA CTG CTG TCA TCC 3’	200

F, forward primer; R, reverse primer; P, probe