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. 2015 May 28;10(5):e0128014. doi: 10.1371/journal.pone.0128014

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© 2015 Hwang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Spreading of PVX-GFP in eEF1B subunit-silenced plants and TRV::00 control plants. Fluorescence was visualized at 7 dpi in the inoculated (a-d) and uninoculated leaves (e-h). Green fluorescence signal indicates presence of GFP-fused PVX and chloroplasts are revealed by red autofluorescence. Scale bars = 50 μm. (B) Accumulation levels of PVX in gene-specific silenced plants. Accumulation of PVX in the inoculated and uninoculated leaves was tested by DAS-ELISA 5 days after PVX infection. This time point corresponds to 18 days after TRV agroinfiltration. Nine plants were tested in 3 independent repeats, with 3 biological plants for each treatment. The error bars indicate standard error. Asterisks denote significant differences between TRV::00 and eEF1B-silenced plants (unpaired t-test: **P <0.01).