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. 2015 May 28;10(5):e0128014. doi: 10.1371/journal.pone.0128014

Fig 4. Protein-protein interaction between elongation factors and viral components.

Fig 4

(A) Interaction between C. annuum eEF1A and eEF1B subunits in the yeast two-hybrid system. (B) Interaction between C. annuum eEF1s and PVX components in the yeast two-hybrid system. Individual transformants were grown on SC-Leu-Try-His+3AT (10 mM) plates for 9 (A) or 6 days (B). pDEST22::Cop1 and pDEST32::Co (Cop1 + Co) were used as positive controls, and empty vector (pDES32) transformants were used as negative controls. (C) Representative images show the BiFC assay results using N. benthamiana epidermal cells. The split bZIP protein (bZIP-YN+bZIP-YC) was used as a positive control. YFP fluorescence generated by protein-protein interaction was detected 2 days after infiltration. Scale bars = 50 μm. (D) Co-IP between C. annuum eEF1s and PVX TGBp1 proteins. Anti-HA-agarose beads were used for immunoprecipitation (IP). Immunoblotting (IB) was performed with anti-HA for elongation factors and anti-FLAG for PVX TGBp1 proteins. GFP-FLAG was used as a negative control.