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. 2015 May 28;10(5):e0127776. doi: 10.1371/journal.pone.0127776

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© 2015 Chintala et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (a) Western blot analysis performed by using antibodies against DREAM and TATA binding protein indicate that DREAM expressed constitutively in retinal nuclear proteins extracted from PBS-treated eyes was reduced in proteins extracted from NMDA-treated eyes. (b) Semi-quantitative analysis of western blots indicate a significant decrease in DREAM levels in retinal protein extracted from NMDA-treated eyes when compared to PBS-treated eyes. *p<0.05. (c) EMSA assays show that the binding of DREAM to DRE oligonucleotides was decreased in retinal nuclear proteins extracted from NMDA-treated eyes (horizontal arrow), when compared to PBS-treated eyes.