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. 2015 May 28;10(5):e0127776. doi: 10.1371/journal.pone.0127776

Fig 5. Characterization of the loss of amacrine cells.

Fig 5

(a) Retinal cross sections prepared at 12 h, 24 h, and 48 h after treating the eyes with NMDA or PBS were immunostained with antibody against calretinin, and their number was quantified (b). Results presented in the figure indicate that when compared to the Calretinin-positive amacrine cells in retinal cross sections prepared from PBS-treated eyes (panels A, B, C), the number of amacrine cells was reduced in the retinal cross sections prepared from NMDA-treated eyes at 12 h (panels D, E, F), 24 h (panels G, H, I), and 48 h (panels J, K, L) after the treatment. (b) Quantification of the calretinin-positive cells indicate that the number of amacrine cells was significantly reduced after NMDA-treatment. *, **p<0.05. NS, not significant. Bar indicates 50 microns size.