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. 2015 May 28;10(5):e0127776. doi: 10.1371/journal.pone.0127776

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© 2015 Chintala et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — Whole retinas (panel A) or retinal cross sections (panels B and C) prepared 24 h after treating the eyes with PBS, PBS plus MK801, NMDA, and NMDA plus MK801 were immunostained with antibodies against Brn3a (panel A), Calretinin (panel B), and PKC-alpha (panel C), quantified by using Nikon elements AR software. Results presented in the figure indicate that NMDA promoted significant loss of Brn3a-positve RGCs, Calretinin-positive amacrine cells, and PKC-alpha-positive bipolar cells. *p<0.05. In contrast, treatment of eyes with NMDA along with MK801 significantly attenuated the degeneration of all three cell types. **p<0.05. NS, not significant.