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. 2015 May 28;10(5):e0119169. doi: 10.1371/journal.pone.0119169

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© 2015 Moriyama et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) The first isolation of MRJP3 with Mono S cation-exchange HPLC. MRJP3 is eluted at 0.20 to 0.30 M NaCl gradient. (B) The second isolation of MRJP3 with Mono Q anion-exchange HPLC. MRJP3 is eluted at 0.15 to 0.30 M NaCl gradient. A dashed square in each chromatograph indicates a elution peak of target protein. The dashed line indicated those recovering fractions on each step.