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. 2015 May 28;11(5):e1005268. doi: 10.1371/journal.pgen.1005268

Fig 4. mec-3 regulatory sequences from distantly-related nematodes drive expression in C. elegans.

Fig 4

(A-D) C. elegans mec-3 regulatory sequence drives expression of mCherry in all transgenic strains; (A) C. briggsae, (B) M. hapla, (C) B. malayi, (D) T. spiralis mec-3 regulatory sequences drive expression of GFP. Animals were photographed at 400x magnification. Images are false-colored composites of single animals. Separate GFP and mCherry images are shown in S3 Fig. (E) Percentage of animals with 0 (white), 1 (light), 2 (mid-tone), or in some cases 3 (darkest shade) cells with reporter gene expression of GFP directed by the heterologous elements and mCherry directed by the C. elegans element. The data are based on counting over 100 individuals carrying each transgene pair (see Materials and Methods and S1 Table for total counts). The C. elegans data are derived from averaging the number of animals expressing mCherry across all mec-3 carrying strains shown here (see Materials and Methods and S1 Table). A maximum of two cells for the FLPs and PLMs and three cells for AVM+ALMs and PVM+PVDs could have expression.