Table 2.
Differential expression of A. gambiae miRNAs in response to P. falciparum at 18 and 24 hr post-infection.
18 hr | Log2 FC | −Log10 p value |
---|---|---|
aae-miR-276 | 4.55 | 1.20 |
aae-miR-285 | 2.38 | 0.38 |
aae-miR-2946 | −2.41 | 0.48 |
aga-miR-11 | −2.75 | 0.70 |
aga-miR-989 | 1.74 | 3.31 |
cqu-miR-210 | 2.54 | 0.38 |
cqu-miR-2951 | −3.10 | 0.69 |
cqu-miR-92 | 2.06 | 0.32 |
24 hr | Log2 FC | −Log10 p value |
---|---|---|
aae-miR-2946 | 2.85 | 0.68 |
aae-miR-34* | −0.14 | 0.35 |
aga-bantam | 2.42 | 0.63 |
aga-miR-11 | 2.23 | 0.61 |
aga-miR-305 | 7.24 | 7.13 |
aga-miR-317 | 2.06 | 0.31 |
aga-miR-989 | 2.42 | 0.40 |
cqu-miR-2951 | 2.75 | 0.66 |
A custom Agilent miRNA microarray was used to compare the miRNA expression profile of A. gambiae midguts at 18 and 24 hr after P. falciparum infection to that of mosquitoes fed on non-infected human blood. Log2FC values were determined from the mean expression value across three biological replicates, following background subtraction and normalization to the 75th percentile. P-values were determined using a moderated t-test. Only miRNAs detected above background level in at least two of the three biological replicates are shown.