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. Author manuscript; available in PMC: 2016 Mar 20.
Published in final edited form as: Science. 2015 Mar 20;347(6228):1374–1377. doi: 10.1126/science.aaa2361

Fig. 2. SIRT7 limits mitochondrial activity, proliferation, and PFSmt.

Fig. 2

(A and B) Increased expression of GFM2 and MRPL24 in SIRT7 KD cells is abrogated by NRF1 siRNA. (C and D) Increased mitochondrial mass in SIRT7 KD cells determined by MTG staining and mitochondrial DNA quantification. n = 3. (E) Increased proliferation in SIRT7 KD cells. n = 3. (F) PFSmt induces SIRT7 expression. Dox, doxycycline. EB, ethidium bromide. (G) Increased accumulation of misfolded ΔOTC in SIRT7 KD cells is rescued by NRF1 siRNA. OTC is used as a control. (H) Increased apoptosis in SIRT7 KD cells treated with EB. n = 3. (I) NRF1 siRNA abrogates increased PFSmt in SIRT7 KD cells. (J) A proposed model. Error bars, mean ± SE. *P < 0.05. **P < 0.01. Student’s t test.