(a) Wild-type (WT) and villin-gp130Act (Tg) small intestine lengths at 3 months (n=5). Data represent averages ± SEM. *P < 0.05. (b) Hematoxylin and eosin (H&E) staining of paraffin-embedded small intestinal sections from WT and Tg mice. Shown are representative images. (c, e–g) Immunohistochemical (IHC) analysis of paraffin-embedded small intestinal sections from WT and Tg mice (n=6). Ki67 and BrdU incorporation (c), Alcian blue (AB)+MMP7 and chromogranin A (ChrA) (e), Wheat germ agglutinin (WGA) (f), and alkaline phosphatase (AP) and DAPI (g) stainings. (d) Ki67 and BrdU positive cells were counted in each crypt. Data are averages ± SD. *P < 0.05. Scale bars represent 100 μm (b, c, e–g).