Extended Data Figure 7. SFK activity is required for YAP activation.

(a) Tg mice (n=4/group) were treated with PP2 (5 mg/kg) or vehicle once a day for 5 days. Small intestines were isolated, sectioned and stained as indicated. Positive cells were enumerated in each villus or crypt. Data represent averages ± SD. *P < 0.05. (b,c) WT and villin-gp130^Act small intestinal organoids were treated with DMSO, AZD0530 (10 μM) (b), AZD1480 (1 μM) or DBZ (10 μM) (c) for 24 hrs, stained with YAP antibody and counter stained with DAPI and photographed under a fluorescent microscope. (d) WT and villin-gp130^Act small intestinal organoids were treated with DMSO, PP2 (10 μM) and AZD1480 (1 μM) for 24 hrs. Total cell lysates were subjected to IB analysis with the indicated antibodies. (e) Serum-starved HT29 cells were pre-treated for 1 hr with 0.1% DMSO (vehicle control), AZD1480 (10 μM) or PP2 (20 μM) prior to IL-6 (10 ng/ml) stimulation for 24 hrs. Nuclear extracts of HT29 cells treated without or with IL-6 in the absence or presence of AZD1480 or PP2 were subjected to IB analysis with the indicated antibodies. Lamin A, a nuclear marker; Actin, a loading control. (f) WT and villin-gp130^Act small intestinal organoids were treated with DMSO and AZD0530 (10 μM) for 24 hrs. Total cell lysates were subjected to IB analysis with the indicated antibodies. Scale bars represent 100 μm (a–c). All data are representative of at least 2–3 independent experiments.