Fig 5. Western blot of Hif1a protein in the liver at 6 weeks (6W) and 6 Months (6M) in Sham (S), Portacaval shunt (PCS), and Portal vein Ligation (PVL) rats.
Nuclear (NUCL) and cytoplasmic (CYT) protein were fractioned and assayed for Histon 3 to serve as a nuclear marker and Actin to serve as a protein marker. There is no staining for Hif1a in each 6 weeks and 6 months groups.