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. 2015 May 28;10(5):e0127668. doi: 10.1371/journal.pone.0127668

Fig 1. Expression pattern of CYPJ gene and its upregulation in HCC.

Fig 1

(A) Northern blot analysis of CYPJ in 16 main human tissues. (B) Northern blot analysis of CYPJ expression in 16 paired HCC/adjacent liver tissues and two fetal liver tissues. (C) Semi-quantitative RT-PCR analysis of CYPJ expression in 40 paired HCC/adjacent tissues. (D) Stage plots of deregulated CYPJ levels in 56 paired HCC/adjacent liver tissues. CYPJ levels were determined by Northern blot/RT-PCR. β-actin was used as internal control, and DR values were calculated. (E) Western blot of CYPJ expression in 15 paired HCC/adjacent normal tissues using anti-PPIL3 antibody. β-actin was used as internal control. Fold change of CYPJ expression in tumor = (T/TC)/(N/NC). T: tumor tissue sample. N: adjacent normal tissue sample. TC: tumor sample control. NC: normal tissue sample control.