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. 2015 May 28;10(5):e0127668. doi: 10.1371/journal.pone.0127668

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© 2015 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Effects of CYPJ and CYPJ(R44A&F49A) overexpression on cell cycle distribution of SK-Hep1 cells. ** P<0.01, N = 3. (B) Knockdown of exogenously enforced CYPJ expression by three different short interference RNAs. The most effective siRNA complex si-J-1 was selected for further analyses. (C) Knockdown of endogenous CYPJ expression by short interference RNA. The expression of CYPA was not affected by the si-J-1 transfection. β-actin was used as internal control. (D) Effects of CYPJ knockdown on cell cycle distribution of SK-Hep1 cells. ** P<0.01, N = 3. (E) Effects of CsA inhibition on cell cycle distribution of SK-Hep1 cells. Cells were treated with 4 μM CsA for 48 h before the experiment. ** P<0.01, N = 3.