Fig 2. Exploratory walking senescence in daGAL4/UAS-InR^DN male and female flies.

(A-B) Representative images of the exploratory walking track of an individual w^Dah control female fly at 1 week old (A) and 7 weeks old (B) during a 15 minute observation period. (C) Survival of daGAL4/UAS-InR^DN once mated female flies compared to daGAL4/+ and UAS-InR^DN/+ controls. Median lifespans and sample sizes were: daGAL4/UAS-InR^DN = 56 days, N = 69; daGAL4/+ = 48 days, N = 99; and UAS-InR^DN/+ = 44 days, N = 60. Survival curves were compared using nonparametric log rank tests and p values calculated. daGAL4/UAS-InR^DN showed an increased survival compared to both controls (P<0.0001). (D-M) Exploratory walking senescence for a cohort of female flies of the indicated genotypes run in parallel with the survival experiment shown in (C). Data are shown as mean value for each parameter ±SEM, N = 12 for the indicated genotype. (D) Female mean distance walked (mm) vs age. (I) Female Total Function of mean distance walked (mm). (E) Female Mean velocity (mm/sec) vs age. (J) Female Total Function of mean velocity (mm/sec). (F) Female Mean walking duration (secs) vs age. (K) Female Total Function of mean walking duration (secs). (G) Female Mean frequency of rotations (change in walking direction) vs age. (L) Female Total Function of mean Rotation Frequency. (H) Female Mean Duration in Central Zone (secs) vs age. (M) Female Total Function of mean duration in central Zone (secs). (N-W) Exploratory walking senescence for a cohort of male flies of the indicated genotypes run in parallel with the survival experiment shown in Fig 1G. Data are shown as mean value for each parameter ±SEM, N = 12 for the indicated genotype. (N) Male mean distance walked (mm) vs age. (T) Male Total Function of mean distance walked (mm). (O) Male Mean velocity (mm/sec) vs age. (T) Male Total Function of mean velocity (mm/sec). (P) Mean walking duration (secs) vs age. (U) Male Total Function of mean walking duration (secs). (Q) Mean frequency of rotations (change in walking direction) vs age. (V) Male Total Function of mean Rotation Frequency. (R) Male Mean Duration in Central Zone (secs) vs age. (W) Total Function of mean duration in central Zone (secs). Data were analysed by two way ANOVA and genotype and age found to be the main effects (p<0.05). Data at individual time points or total function data were analysed by one way ANOVA followed by post hoc means comparisons using Tukey HSD, and * indicates significant differences (p<0.05) between daGAL4/UAS-InR^DN flies and both controls.