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. 2015 May 28;10(5):e0127465. doi: 10.1371/journal.pone.0127465

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© 2015 Gürke et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — The relative amount of mTOR phosphorylation was quantified by Western blotting in four samples pooled from a total of 30 blastocysts of gastrulation stage 2. (A) shows a representative Western Blot of two pooled samples (n≥ 7 blastocysts per sample) from blastocysts from control (lanes 1,2) and diabetic (lanes 3,4) rabbits. The amount of phosphorylated protein was related to the non-phosphorylated protein and calculated relative to the healthy blastocysts (set 1). The phosphorylation of mTOR was significantly increased in stage 2 blastocysts of diabetic rabbits compared to controls (B). (N = 4; n = 30; ** = p< 0.005).