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. 2015 May 28;11(5):e1005238. doi: 10.1371/journal.pgen.1005238

Fig 5. Disruption of miR-29 expression in vivo leads to aberrant vSMC differentiation.

Fig 5

(A&B) Double IF staining of α-SMA and COL1A1; significantly reduced α-SMA staining (green) in distal vasculature was observed in miR-29 null lungs (DKO) as compared to wild type littermate controls (WT). This reduced expression of α-SMA is associated with upregulation of COL1A1(red), a known direct target of miR-29. Images are representative of four pairs of littermate-matched WT/DKO. (C) Immunoblotting analysis of α-SMA, MYOCD, TAGLN and MYH11 of whole lung protein extracts of three littermate-matched WT/DKO pairs. (D) Densitometric analyses of the blots are presented as relative ratios of specific protein/GAPDH. Ratio of the WT control is arbitrarily presented as 1. Data are mean ±SEM from 3 experiments.