Fig 2. Antioxidant enzymes in DEF vs. AI G93A mice.

SOD2 (A and B), catalase (C and D) and GPx1 (E and F) protein content (arbitrary units; AU) in spinal cord of 42 G93A mice: 23 adequate vitamin D₃ intake (AI; 1 IU D₃/g feed; 12 M, 11 F) and 19 deficient vitamin D₃ intake (DEF; 0.025 IU D₃/g feed; 10 M, 9 F). SOD2 (A and B): DEF males had 18% lower SOD2 protein content vs. AI males (P = 0.034). DEF males had 27% lower SOD2 protein content vs. DEF females (P = 0.004). Catalase (C and D): There was no significant difference in catalase protein content between the diets or between the sexes. GPx1 (E and F): DEF mice had 12% higher GPx1 protein content vs. AI (P = 0.057). DEF females had 29% higher GPx1 protein content vs. AI females (P = 0.001). AI males had 10% higher GPx1 protein content vs. AI females (P = 0.054). DEF males had 17% lower GPx1 protein content vs. DEF females (P = 0.070). Data presented as means ± SEM.