Fig 3. Inflammation in DEF vs. AI G93A mice.

TNF- α (A and B), IL-6 (C and D) and IL-10 (E and F) protein content (arbitrary units; AU) in spinal cord of 42 G93A mice: 23 adequate vitamin D₃ intake (AI; 1 IU D₃/g feed; 12 M, 11 F) and 19 deficient vitamin D₃ intake (DEF; 0.025 IU D₃/g feed; 10 M, 9 F). TNF-α (A and B): There was no significant difference in TNF-α protein content between the diets or between the sexes. IL-6 (C and D): DEF females had 22% higher IL-6 protein content vs. AI females (P = 0.077). AI males had 14% lower IL-6 protein content vs. AI females (P = 0.075). DEF males had 29% lower IL-6 protein content vs. DEF females (P = 0.023). IL-10 (E and F): There was no significant difference in IL-10 protein content between the diets. AI males had 11% lower IL-10 protein content vs. AI females (P = 0.074). Data presented as means ± SEM.