Fig 1. Schematic representation of (A) the pWPXL-Ova-HNP4 and (B) pWPXL-Ova-HNP4-His lentiviral vectors used for oviduct-specific expression in transgenic chickens.

LTR, long terminal repeat; ψ, packing signal; RRE, rev response elements; cPPT, central polypurine tract; chicken lysozyme signal peptide (area in black); HNP4, human neutrophil defensin 4; Probe, probe used for Southern blotting; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element. The locations of Primer-F and Primer-R (black arrows) used for screening the transgenic chickens are shown, and the positions of the restriction site Bgl II and EcoR I used for Southern blot analysis are marked. The figure is not drawn to scale.