Fig 2. Expression and antimicrobial activity of recombinant HNP4 at the cellular level.

A. RT-PCR analysis of the HNP4 transcripts expressed in transfected 293T cells. Lanes 1–4 represent the 293T cells transfected with the pBud-GFP-HNP4-His vector and lane 5 represents the wild-type 293T cells. B. Western blot analysis of HNP4 protein expression in transfected 293T cells. The results obtained from these samples were consistent with those obtained using samples assayed by RT-PCR. C. Antimicrobial activity of the recombinant HNP4 in vitro. The total protein was extracted from the 293T cells transfected with the pBud-EGFP-HNP4-His plasmid, and the HNP4-His protein was purified from the total protein. Ampicillin was used as a positive control at a concentration of 50 μg/ml, and H₂O was used as a negative control.