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. 2015 May 28;10(5):e0127922. doi: 10.1371/journal.pone.0127922

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© 2015 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — A. PCR analysis of the G₁ transgenic chickens. Lane 1, Marker; Lanes 2–16, DNA samples isolated from the blood of G₁ transgenic chickens; Lane 17, DNA sample isolated from the blood of nontransgenic chickens; Lane 18, positive control (Ova-HNP4-His plasmid). B. Southern blot analysis of the G₁ transgenic chickens. Genomic DNA samples extracted from the G₁ transgenic and wild-type animals were digested with Bgl II to confirm the integration of the transgene. Lanes 1–15, DNA samples from the blood of the G₁ transgenic chickens; Lane 16, DNA sample from the blood of a wild-type chicken. C. Copy number analyzed with Southern blotting. The genomic DNA samples were digested with EcoR I to confirm the copy number in the G₁ transgenic chickens. The results showed six different locations of insertion, and all the G₁ chickens carried a single copy of the transgene. Lanes 1–15 were the DNA samples extracted from the blood of the G₁ transgenic chickens. D. Genome walking analysis of the insertion sites. The results indicated that a single copy of the transgene was integrated into chromosomes 1, 2, 3, 4, 6 and 24 of the six chickens.