Fig 5. Analysis of the expression of the HNP4 and HNP4-His genes at the translational level.

A. Immunofluorescent detection of HNP4 in oviduct sections. HNP4 protein (red) in the oviduct of hen G₁-46 was visualized in a section of the magnum portion of the oviduct by staining with the HNP4 antibody; the oviduct sections were also stained with the chicken lysozyme antibody (CLY) as a positive control. The oviduct sections of the wild-type (WT) chickens were stained with the HNP4 antibody, but no signal was observed. B. Yields of HNP4 in the egg whites of the G₁ and G₂ hemizygous chickens. The egg white solution was assayed by ELISA. The quantities of HNP4 varied greatly in the hens with different insertions, ranging from 1.65 μg/ml to 10.18 μg/ml.