Fig 5. Production of hyaluronan by chondrocytes in alginate and chitosan-alginate beads.

Hyaluronan was quantified in CM (cellular matrix), FRM (further-removed matrix) and culture supernatant after key intervals throughout the culture time (i.e. 3, 21, 28 days). Productions were expressed in absolute values (ng) and normalized to DNA content. Results were presented as mean ± SEM of 4 independent experiments (n = 4). Statistical significance was determined after one way ANOVA, followed, if positive, by the Bonferroni’s multiple comparison post-test.