Table 2. Production of IL-6, IL-8, PGE2 and MMP-3 by chondrocytes in A and CA beads after key intervals throughout the culture time: 0–3, 17–21, 24–28 days.
| 0–3 | 17–21 | 24–28 | ||
|---|---|---|---|---|
| IL-6 | A (pg/μg DNA) | 8,153 (4,286–20,592) | 695 (95–1,484) | 229 (1–460) |
| CA (pg/μg DNA) | 2,530 (1,689–6,750) | 229 (114–573) | 147 (99–395) | |
| % of alginate control | 22 (1–43) ** | 28 (5–63) ** | 78 (13–169) | |
| IL-8 | A (pg/μg DNA) | 30,286 (13,757–74,328) | 2,404 (116–4,691) | 501 (156–845) |
| CA (pg/μg DNA) | 11,773 (10,964–34,509) | 197 (68–463) | 108 (18–235) | |
| % of alginate control | 39 (4–74) *** | 8 (3–19) *** | 21 (2–43) *** | |
| PGE2 | A (pg/μg DNA) | 10,894 (411–22,199) | 354 (331–1,040) | 105 (7–217) |
| CA (pg/μg DNA) | 3,153 (261–6,568) | 320 (229–869) | 84 (32–201) | |
| % of alginate control | 31 (21–41) ** | 102 (9–195) | 82 (36–129) | |
| MMP-3 | A (ng/μg DNA) | 3,984 (5,002–12,970) | 1,727 (1,686–5,140) | 460 (109–810) |
| CA (ng/μg DNA) | 2,328 (3,523–8,179) | 498 (59–1,056) | 335 (65–605) | |
| % of alginate control | 46 (29–64) ** | 50 (19–119) ** | 73 (53–92) |
Production of IL-6, IL-8 PGE2 and MMP-3 by chondrocytes in A and CA beads. IL-6, IL-8, PGE2and MMP-3 were quantified in cell culture supernatants after key intervals throughout the culture time (i.e. 0–3, 17–21, 24–28 days). Productions were normalized to DNA content. Results were expressed as concentration per μg DNA of chondrocyte production in A and CA beads, and as the percentage of chondrocyte production in A beads (100% = control). Results were expressed as mean (95% confidence interval) of 4 independent experiments (n = 4). Statistical significance in comparison to production in A beads (100% = control) was determined after one way ANOVA, followed, if positive, by the Bonferroni’s multiple comparison post-test: A vs CA: *** p<0.001; ** p<0.01.