Fig 5. Nanotrap particles can capture and enrich NP from viral supernatants.

A) Supernatants from mock infected or MP12 infected (MOI 0.1, 1, or 10) Vero cells were collected at 24 hours post infection. One milliliter of supernatants were incubated with 100 μl of NT45 for 30 minutes. Bound materials were analyzed for presence of NP by western blot using antibodies against NP. No Nanotrap particle (-NT) and mock-infected samples containing no Nanotrap particles (10 μl volumes) were processed in parallel. Control sample is CE at 770 μg/ml (10 μL volume). B) Supernatants from mock infected or MP12 infected (MOI 10) Vero cells were collected at 8, 16, and 24 hours post infection. One milliliter of supernatants were incubated with 100 μl of NT45 for 30 minutes. The samples were processed as in panel A. C) Viral supernatants collected at 48 hours post infection were diluted to a viral titer of 1E+06 pfu/ml. A volume of 100 μl, 500 μl, and 1000 μl were incubated with 100 μl NT45 for 30 minutes. The samples were processed as in panel A. The control sample is viral supernatant at 1E+07 pfu/ml (10 μl volume). D) Viral supernatants (collected 48 hours post infection) at 1E+07 pfu/ml, 1E+06 pfu/ml, 1E+05 pfu/ml, and 1E+04 pfu/ml were incubated at 1 ml volumes with 100 μl of NT45 for 30 minutes. The samples were processed as in panel A.