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. 2015 May 28;10(5):e0128430. doi: 10.1371/journal.pone.0128430

Fig 4. Immunoprecipitation of the gradient purified 45S and 35S complexes using anti-DDX35 antibodies.

Fig 4

The fractions from the gradient analysed in Fig 3, corresponding to the 35S region (fractions 9–12—lane 3 and 4) or the 45S region (fractions 14–17—lane 1 and 2), were combined and subjected to immunoprecipitation with the anti-DDX35 antibodies (lane 1 and 3) or with the anti-DDX35 antibodies pre-blocked with antigenic peptide (lanes 2, 4). The RNA, extracted from the beads, was labelled with 32P-pCp and analysed by denaturing PAGE followed by autoradiography. Identities of the RNA species are shown on the left. The bands marked 5.8S and 5S correspond to rRNA that was precipitated unspecifically.