Fig 2. miR-22 overexpression results in hair loss.

(A) Schematic maps of constructs used to generate K14-rtTA/TRE-miR-22 double transgenic mice (DTG). (B) qPCR analysis for miR-22 showing that miR-22 is strongly induced in the Dox-treated DTG mice. The DTG mice were treated with Dox at P21. Samples were collected at P28. ** p < 0.01. (C) The Dox-treated DTG mice exhibit hair loss at 35 days. Both control and DTG mice were treated by oral administration of 2 mg/mL Dox in the drinking water at P1. After removing Dox at P35, the hair loss phenotype recovers by P46. (DTG n = 3; control n = 3). (D) Hair loss in DTG mice at P49 when treated with Dox at P1. (E) External hair regrowth is delayed in the DTG mice after depilation. Both control and DTG mice were treated with Dox at P18. Hairs were plucked at P21. Photographed at P31 and P40. (DTG n = 21; control n = 16).