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. 2015 May 28;11(5):e1005253. doi: 10.1371/journal.pgen.1005253

Fig 7. Identification of direct miR-22 target genes.

Fig 7

(A) Heatmaps of transcription factors downregulated in DTG skin. (B) qPCR for Foxn1, Dlx3, Sostdc1 and Hoxc13 in control and miR-22 KO skin at P18. * p < 0.05; ** p < 0.01. (C) Immunofluorescence for Foxn1, Dlx3, Sostdc1 and Hoxc13 in control and DTG skin. (D) Representative immunoblot for Foxn1, Dlx3, Sostdc1 and Hoxc13 proteins in control and DTG skin. Tubulin was used as a control. (E) Luciferase reporter activity of Foxn1, Dlx3, Sostdc1 and Hoxc13 wild type and mutant 3’UTR constructs based on 3 independent experiments. ** p < 0.01; * p < 0.05.