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. 2015 May 28;10(5):e0127935. doi: 10.1371/journal.pone.0127935

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© 2015 Nascimento et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — Melting curves (dissociation curves) of the 13 reference genes amplicons after the RT-qPCR reactions, all showing one peak. Melting, amplification and standard curves for mitochondrially encoded cytochrome c oxidase II (MT-CO2) target gene are also showed.