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. 2015 May 28;10(5):e0127935. doi: 10.1371/journal.pone.0127935

Fig 5. Relative expression of this MT-CO2 target gene when normalized with poor ranked reference genes and the best reference genes using Pfaffl method.

Fig 5

This Figure shows the effect of incorrect selection of reference gene in the gene expression of target gene. Two best ranked reference genes (HMBS and HPRT1) and the two poor ranked reference genes (TFRC and B2M). The results also include standard errors (SE_R) and two-tailed P values by Student's t test. A single asterisk indicates P-value < 0.05.