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. 2015 May 28;10(5):e0127628. doi: 10.1371/journal.pone.0127628

Fig 1. Human urothelial cultures release MIF after activating PAR1 or PAR4.

Fig 1

Human benign transformed urothelial cells (UROtsa) were exposed to specific human PAR1 (TFLLR-NH2) or PAR4 (AYPGKF-NH2) activating peptides (AP), or respective scrambled peptides (control). Culture media was collected at 15, 60, and 120 minutes after AP application and assayed for MIF using a commercial ELISA kit (R&D Systems; Minneapolis, MN). Significant urothelial MIF release occurring within the first 15 minutes was observed after PAR1 or PAR4 stimulation either at 25 μM (A) or 50 μM (B) concentrations. * p ≤ 0.05.