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. 2015 May 28;10(5):e0124106. doi: 10.1371/journal.pone.0124106

Fig 5. The functional characterization of SfCPS and SfKSL in E. coli.

Fig 5

A) SfCPS characterization. (a) GC-MS profile (275 m/z extracted ion chromatograms) of the dephosphorylated product of mature SfCPS incubated with GGDP. (b) GC-MS of the control reaction- substrate GGDP omitted. (c) Mass spectrum of the product peak- copalol. B) SfKSL characterization. (a) GC-MS profile (272 m/z extracted ion chromatograms of the product (miltiradiene) of coupled reaction of SfCPS and SfKSL with GGDP as substrate, b) GC-MS profile of the enzymatic assay with SfCPS omitted. c) Mass spectrum of the reaction product—miltiradiene.