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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Cell Physiol. 2015 Sep;230(9):2142–2151. doi: 10.1002/jcp.24943

Figure 3. Effects of TPO stimulation and c-mpl expression on OC formation and resorption.

Figure 3

A) PBMCs, from healthy volunteers, were isolated and cultured with M-CSF (20 ng/ml), RANKL (80 ng/ml), and TPO (0 or 100 ng/ml). Although OCs formed in all specimens tested, treatment with TPO did not alter OC number. B &C) PBMCs were separated into subpopulations based on the expression of either CD11b or CD14, cell surface markers for OC progenitors, and then further separated based on c-mpl (CD110) expression. Representative data from one of the healthy volunteers is shown. B) Few TRAP+ OCs were generated from CD11b+CD110− or CD14+CD110− populations. Numerous TRAP+ OCs were generated from CD11b+CD110+ or CD14+CD110+ populations. C) The activity of the OCs generated from these subpopulations was also assessed. When normalized for the number of TRAP+ OCs, the activity of the OCs (defined as the total resorption pit area on the dentin slice/TRAP+ OCs) was highest in the unsorted PBMC population. Activity was significantly lower in all of the sorted populations examined. Of interest, TPO treatment significantly enhanced the activity, in CD14+CD110+ cells. Due to the limited number of TRAP+ OCs generated from CD11b+CD110− and CD14+CD110− cells (panel B) we were unable to obtain activity data for these populations. D) In another study CD14+ cells were further separated into CD110+, CD110dim, and CD110− populations, cultured as above, and OC number determined. A CD110 expression-dependent increase in OC number was observed.