FIG. 3.

Expresssion analysis of genes bccpr1, bcaba1, and P450-12. (A) Total RNA was extracted from ABA-producing B. cinerea strain ATCC 58025 (lanes +) and nonproducing strain B05.10 grown for 4 days (bccpr1 and bcaba1) or 11 days (P450-12) in Sprecher medium. (B) Total RNA was extracted from strain ATCC 58025 grown for 3 days in Sprecher medium. Mycelia were harvested 30, 60, 90, 120, and 180 min after the addition of 3.8 mM mevalonic acid lactone. Fragments of the 3′ region of bccpr1 (see Fig. 4), the 5′ region of bcaba1 (see Fig. 7), and an internal 720-bp PCR fragment of P450-12 were used for probing. Loading of lanes with RNA was checked by probing with ribosomal DNA.