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Hydroxyproline loss in rat subdermal explants. Quantification of hydroxyproline content by 1H-NMR in 90 day rat subdermal explants. (A) Hydroxyproline content reported/gram dry weight (B) hydroxyproline content/gram dry weight adjusted for calcium and phosphate weight. Control: glutaraldehyde fixed BP, Chemistry: glutaraldehyde fixed BP reacted with EDC and SuOH, DBP: glutaraldehyde fixed BP modified with DBP using EDC and SuOH. n=3-5.
